We herein show that simple supplementation with

2018-10-20

We herein show that simple supplementation with doxycycline greatly improves both hESC/iPSC viability and self-renewal. In practice, doxycycline dramatically enhances hESC/iPSC expandability, and the effects continue for long time periods. The effects of doxycycline are mediated by direct activation

Here we used iPSCs to generate cardiomyocytes

2018-10-20

Here, we used iPSCs to generate cardiomyocytes by reprogramming skin fibroblasts from two patients carrying dissimilar LQTS mutations. Associated with LQT2, HERG-A422T produces trafficking-defective HERG K+ aconitine (Kv 11.1) that decrease the rapidly activating delayed rectifier current (IKr), wh

salinomycin br Introduction Advances in stem cell

2018-10-20

Introduction Advances in stem-cell-based therapies may help overcome CNS disorders such as spinal cord injury (SCI). Transplantation of neural stem/progenitor salinomycin (NS/PCs) has yielded beneficial effects and improved functional recovery in SCI animal models (Cummings et al., 2005; Hofstet

We have previously shown that the

2018-10-20

We have previously shown that the piggyBac (PB) transposon modified from moth can efficiently transpose in the mouse and human genomes (Ding et al., 2005). Here we present a gain of function screen in human ES Tasquinimod using PB transposon mutagenesis. The transposon is specially designed for ide

Although previous work showed that SOX is sufficient to conv

2018-10-20

Although previous work showed that SOX2 is sufficient to convert fibroblasts into NES+ multipotent NSCs under a unique culture condition (Ring et al., 2012), our genetic approach using Nes-CreER;Rosa-YFP mice failed to detect a stable NSC state during the in vivo reprogramming process. This differen

Cryopreserved peripheral blood mononuclear cells PBMC

2018-10-20

Cryopreserved peripheral blood mononuclear cells (PBMC) from this patient were used for the generation of an induced pluripotent stem cell (iPSC) that harbour a “highest risk” RET mutation according to ATA guidelines, i.e. a 2753T>C missense de novo mutation at exon 16 leading to the most frequent M

To trace host to graft connectivity of transplanted hESC der

2018-10-20

To trace host-to-graft connectivity of transplanted hESC-derived neurons, we next generated hESC lines that stably expressed the tracing and control constructs (Figures 2A and S2A). The engineered hESCs were differentiated using a protocol that results in a high yield of authentic and functional ven

Sulfo-NHS-SS-Biotin RPE cell associated allografts have been

2018-10-20

RPE cell-associated allografts have been considered for the treatment of ocular diseases such as age-related macular degeneration (AMD). We successfully established human RPE Sulfo-NHS-SS-Biotin from human iPSCs (Kamao et al., 2014; Sugita et al., 2015). In addition, we recently transplanted an iPSC

Hello world!

2018-07-29