br Data These data mainly focus on

Data These data mainly focus on the dose-dependent neuroprotection provided by baclofen, memantine and l-carnitine against oxygen-glucose-deprivation (OGD)-related neurodegeneration of CA1 region, with an addition of the data obtained from the use of hypothermia instead of pharmacological agents. The data consists of CA1 stratum pyramidale (CA1sp) width measurements from slices incubated in OGD medium for 60min with or without neuroprotective applications. The values were normalized with the CA1sp measurements of healthy slices incubated in artificial cerebrospinal fluid (ACSF) for 60min. The dose-response curves for baclofen, memantine and l-carnitine is provided, together with the comparison of the protection levels for most effective doses of single chemical agents to chemical agent combinations or hypothermia.
Experimental design, materials and methods Acute hippocampal slice preparation, slice incubation and histology procedures are described in [1]. The CA1sp was inspected under light microscope (Leica DM 2500, Leica Microsystems Inc., Buffalo Grove, IL, USA) and photographed with 20X magnification (DFC310FX, Leica Microsystems Inc., Buffalo Grove, IL, USA). CA1sp width measurements were carried on these 740 Y-P images using ImageJ software [2]. The details of measurements, estimation and statistics are described in [1] (Fig. 1). Dose-response curves for baclofen (bac, Sigma-Aldrich, St. Louis, MO, USA), Memantine-HCl (mem, Sigma-Aldrich, St. Louis, MO, USA), and (L)-carnitine-HCl (lcar, Sigma-Aldrich, St. Louis, MO, USA) display the mean CA1sp width values for each dose, normalized with the mean CA1sp width from healthy control slices. Error bars represent standard errors. Zero dose represents the OGD medium incubation without any pharmacological application (negative control) (Fig. 2). For the comparison of single pharmacological application with combinatory uses and with hypothermia, the normalized CA1sp width measurements from 25mM bac, mem and lcar applications were represented as positive controls. The pharmacological combinations were as follows: (a) bac 10mM+mem 5mM, (b) bac 10mM+mem 10mM, (c) bac 10mM+lcar 5mM, (d) bac 5mM+lcar 5mM. All values were compared to the normalized CA1sp width measurements from slices incubated in OGD medium without any applications (negative control) and slices incubated in ACSF incubation (double negative control) (Fig. 3). Significant differences between groups were assessed using one-way analysis of variance (ANOVA); post-hoc tests further examined between-group differences. Statistical analyses were conducted using SAS Studio 3.5 (SAS Institute Inc., Cary, NC, USA). Tukey׳s post-hoc test was used to compare individual groups amongst themselves (α=0.05).
Acknowledgments The authors would like to thank Ö. Mavuk for her contribution to histology, Associate Professor Dr. B. Güçlü for his technical support, and M. C. Mutlu for his assistance in the experiments. This project was supported by Bogazici University Scientific Research Fund (Grant number 7220). During this study, P. Ö. received a post-doctoral research scholarship from The Scientific and Technological Research Council of Turkey (TUBITAK).
Data The presented 3D dataset illustrates the application of the fast macromolecular proton fraction (MPF) mapping method [1–3] for generation of high-contrast high-resolution images of the rodent brain in vivo in ultra-high magnetic fields. The entire dataset contains 3D image files in the Analyze 7.5 format representing three source images with different contrast weightings (proton density (PD): files “PD.img” and “PD.hdr”, T1: files “T1.img” and “T1.hdr”, and magnetization transfer (MT): files “MT.img” and “MT.hdr”) of the rat brain in vivo acquired on an 11.7T small animal MRI scanner and an MPF map (files MPF.img and MPF.hdr) reconstructed from these images. Each 3D image has isotropic spatial resolution of 170×170×170µm and contains a 200×200×200 matrix with voxel intensities in 16-bit little-endian format. Intensities of PD-, T1-, and MT-weighted images are in arbitrary units and MPF map intensities represent percentage MPF values multiplied by 100. Example reformatted coronal sections of the 3D MPF map are presented in Fig. 1.