Introduction Linear transcriptional pathways that govern

2018-10-20

Introduction Linear transcriptional pathways that govern intestinal tumorigenesis have been extensively explored in mice using gene knock outs (KO) and to a limited extent hypomorphs as well as chemical inhibitors (Aoki and Taketo, 2007; Barker et al., 2009, 2010; Batlle et al., 2005; Cheasley et a

In this study we demonstrated that unrelated

2018-10-20

In this study, we demonstrated that unrelated allogeneic human MSCs supported a LSC phenotype in a largely quiescent state in a long-term vitro culture system. We then confirmed the persistence of LIC after 6weeks co-culture with MSCs using the NSG mouse xenotransplant model. Our results also reveal

Besides IL RCC and RAEB t MDS

2018-10-20

Besides IL6 (RCC- and RAEB(t)-MDS), DKK3 (RCC-MDS), CRLF1 and DAPK1 (RAEB(t)-MDS) were differentially expressed by MSCs of healthy controls versus MDS. DKK3 and CRLF1 have been associated with increased cell survival by suppressing apoptosis in MSCs and neuroblastoma cells, respectively (Song et al.

Several groups have reported the culture

2018-10-20

Several groups have reported the culture and differentiation of fetal or adult neural stem finding molarity (NSCs) on polymer-based substrates (Park et al., 2014; Little et al., 2008; Bhang et al., 2007; Lundin et al., 2011; Saha et al., 2007). It is important to point out that the NSCs used in the

SAMHD emerges as a ubiquitous and potent barrier to

2018-10-20

SAMHD1 emerges as a ubiquitous and potent barrier to productive HIV-1 infection in DCs, myeloid cells, and resting CD4+ T cells. Thus, it appeared likely that SAMHD1 might also be effective when transducing HSPCs with lentiviral vectors. Efficient transduction of HSPCs requires prestimulation with c

trp channels br Materials and methods br Author disclosure

2018-10-20

Materials and methods Author disclosure statement Acknowledgments This study was supported by fund from National Medical Research Council (NMRC) of Singapore, the Translational Clinical Research (TCR) award (NMRC/TCR/013-NNI/2014) and STaR award (NMRC/STaR/014/2013). We thank Ms. Fiona Seti

We herein show that simple supplementation with

2018-10-20

We herein show that simple supplementation with doxycycline greatly improves both hESC/iPSC viability and self-renewal. In practice, doxycycline dramatically enhances hESC/iPSC expandability, and the effects continue for long time periods. The effects of doxycycline are mediated by direct activation

Here we used iPSCs to generate cardiomyocytes

2018-10-20

Here, we used iPSCs to generate cardiomyocytes by reprogramming skin fibroblasts from two patients carrying dissimilar LQTS mutations. Associated with LQT2, HERG-A422T produces trafficking-defective HERG K+ aconitine (Kv 11.1) that decrease the rapidly activating delayed rectifier current (IKr), wh

salinomycin br Introduction Advances in stem cell

2018-10-20

Introduction Advances in stem-cell-based therapies may help overcome CNS disorders such as spinal cord injury (SCI). Transplantation of neural stem/progenitor salinomycin (NS/PCs) has yielded beneficial effects and improved functional recovery in SCI animal models (Cummings et al., 2005; Hofstet

We have previously shown that the

2018-10-20

We have previously shown that the piggyBac (PB) transposon modified from moth can efficiently transpose in the mouse and human genomes (Ding et al., 2005). Here we present a gain of function screen in human ES Tasquinimod using PB transposon mutagenesis. The transposon is specially designed for ide

Although previous work showed that SOX is sufficient to conv

2018-10-20

Although previous work showed that SOX2 is sufficient to convert fibroblasts into NES+ multipotent NSCs under a unique culture condition (Ring et al., 2012), our genetic approach using Nes-CreER;Rosa-YFP mice failed to detect a stable NSC state during the in vivo reprogramming process. This differen

Cryopreserved peripheral blood mononuclear cells PBMC

2018-10-20

Cryopreserved peripheral blood mononuclear cells (PBMC) from this patient were used for the generation of an induced pluripotent stem cell (iPSC) that harbour a “highest risk” RET mutation according to ATA guidelines, i.e. a 2753T>C missense de novo mutation at exon 16 leading to the most frequent M

To trace host to graft connectivity of transplanted hESC der

2018-10-20

To trace host-to-graft connectivity of transplanted hESC-derived neurons, we next generated hESC lines that stably expressed the tracing and control constructs (Figures 2A and S2A). The engineered hESCs were differentiated using a protocol that results in a high yield of authentic and functional ven

Sulfo-NHS-SS-Biotin RPE cell associated allografts have been

2018-10-20

RPE cell-associated allografts have been considered for the treatment of ocular diseases such as age-related macular degeneration (AMD). We successfully established human RPE Sulfo-NHS-SS-Biotin from human iPSCs (Kamao et al., 2014; Sugita et al., 2015). In addition, we recently transplanted an iPSC

Hello world!

2018-07-29